blue fluorescent dna stain dapi  (Thermo Fisher)

Journal: eLife

Article Title: Differential accumulation of storage bodies with aging defines discrete subsets of microglia in the healthy brain

doi: 10.7554/eLife.57495

Figure Lengend Snippet:

Article Snippet: Other , DAPI , ThermoFisher , Cat# 62248 , Cell impermeant blue-fluorescent DNA stain for viability used at a concentration of 0.1 μg/mL.

Techniques: Isolation, Staining, Concentration Assay, Labeling, Software, Gradient Centrifugation, Sequencing, Control, Multiplex Assay, Recombinant, Mass Spectrometry, Modification

Journal: Molecular Biology of the Cell

Article Title: ADP-ribosylation factor–like 4A interacts with Robo1 to promote cell migration by regulating Cdc42 activation

doi: 10.1091/mbc.E18-01-0001

Figure Lengend Snippet: Arl4A induces the localization of Robo1 at the plasma membrane. (A) HeLa cells were transfected with Arl4A-WT (red) or Robo-myc-WT (green) alone. (B) HeLa cells were cotransfected with Arl4A-WT and Robo1-Myc-WT. CD44 staining was used to define the plasma membrane boundary of HeLa cells. Cell nuclei were stained with DAPI. (C) PM/C ratios for Robo-myc-WT in the single-expression and Arl4A coexpression groups. (D) Cellular localization of Arl4A-binding defective mutants (A1 and A2) in the single-expression and Arl4A coexpression groups. (E) Quantitative data from A, B, and D. Scale bar = 10 µm. The numbers of analyzed cells are shown in each column. The solid bars represent the mean ± SD. **, P < 0.005; ***, P < 0.001 (C: Student's t test; E: one-way ANOVA with Dunnett's post hoc multiple comparison test).

Article Snippet: A blue-fluorescent DNA stain 4′,6-diamidino-2- phenylindole (DAPI) solution was purchased from Millipore (1:5000, catalogue no. S7113).

Techniques: Clinical Proteomics, Membrane, Transfection, Staining, Expressing, Binding Assay, Comparison